sirtuin 3 antibody Search Results


anti sirt3  (Novus Biologicals)
90
Novus Biologicals anti sirt3
Anti Sirt3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirtuin 3/sirt3 antibody  (Bio-Techne corporation)
90
Bio-Techne corporation sirtuin 3/sirt3 antibody
Sirtuin 3/Sirt3 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sirtuin 3/sirt3 antibody - by Bioz Stars, 2026-03
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sirtuin 3 (em0731  (Elabscience Biotechnology)
90
Elabscience Biotechnology sirtuin 3 (em0731
Sirtuin 3 (Em0731, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirtuin 3 (em0731 - by Bioz Stars, 2026-03
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sirt3  (Boster Bio)
90
Boster Bio sirt3
Primers used for human reverse transcription-quantitative polymerase chain reaction in the present study.
Sirt3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Primers used for human reverse transcription-quantitative polymerase chain reaction in the present study.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: Primers used for human reverse transcription-quantitative polymerase chain reaction in the present study.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Polymerase Chain Reaction, Sequencing

Primers used for mouse reverse transcription-quantitative polymerase chain reaction in the present study.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: Primers used for mouse reverse transcription-quantitative polymerase chain reaction in the present study.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Polymerase Chain Reaction, Sequencing

mRNA expression levels of SIRTs determined by reverse transcription-quantitative polymerase chain reaction in (A) cells, (B) mouse tissue and (C) human tissue. The expression levels of SIRT1, SIRT2, SIRT3, SIRT4 and SIRT5 in the experimental groups/patients with psoriasis were significantly reduced and those of SIRT6 and SIRT7 were significantly increased compared with the control groups. Error bars present the standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control group. SIRT, Sirtuin.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: mRNA expression levels of SIRTs determined by reverse transcription-quantitative polymerase chain reaction in (A) cells, (B) mouse tissue and (C) human tissue. The expression levels of SIRT1, SIRT2, SIRT3, SIRT4 and SIRT5 in the experimental groups/patients with psoriasis were significantly reduced and those of SIRT6 and SIRT7 were significantly increased compared with the control groups. Error bars present the standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control group. SIRT, Sirtuin.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Standard Deviation

Protein expression levels of SIRTs determined by western blotting in (A) cells, (B) mouse tissue and (C) human tissue. The protein expression levels of SIRT1, SIRT2, SIRT3, SIRT4 and SIRT5 in the experimental groups/patients with psoriasis were significantly reduced and those of SIRT6 and SIRT7 were significantly increased compared with the controls. Error bars present the standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control group. SIRT, Sirtuin.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: Protein expression levels of SIRTs determined by western blotting in (A) cells, (B) mouse tissue and (C) human tissue. The protein expression levels of SIRT1, SIRT2, SIRT3, SIRT4 and SIRT5 in the experimental groups/patients with psoriasis were significantly reduced and those of SIRT6 and SIRT7 were significantly increased compared with the controls. Error bars present the standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control group. SIRT, Sirtuin.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Expressing, Western Blot, Standard Deviation, Control

IF in human skin lesions. IF in human psoriasis skin lesions was negative for (A) SIRT1, (B) SIRT2, (C) SIRT3, (D) SIRT4 and (E) SIRT5 antibodies. Lesions were positive for (F) SIRT6 and (G) SIRT7 antibodies. SIRTs were mainly observed in the epithelial layer. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: IF in human skin lesions. IF in human psoriasis skin lesions was negative for (A) SIRT1, (B) SIRT2, (C) SIRT3, (D) SIRT4 and (E) SIRT5 antibodies. Lesions were positive for (F) SIRT6 and (G) SIRT7 antibodies. SIRTs were mainly observed in the epithelial layer. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Immunofluorescence

IF in mouse skin lesions. IF in mouse skin lesions were also negative for (A) SIRT1, (B) SIRT2, (C) SIRT3, (D) SIRT4 and (E) SIRT5 antibodies. Lesions were positive for (F) SIRT6 and (G) SIRT7 antibodies. SIRTs were mainly localized in the epithelial layer. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: IF in mouse skin lesions. IF in mouse skin lesions were also negative for (A) SIRT1, (B) SIRT2, (C) SIRT3, (D) SIRT4 and (E) SIRT5 antibodies. Lesions were positive for (F) SIRT6 and (G) SIRT7 antibodies. SIRTs were mainly localized in the epithelial layer. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Immunofluorescence

IF in TNF-α-stimulated HaCaT cells was negative except for SIRT6 and 7. (A) SIRT1 and (B) SIRT2 were located predominantly in the nucleus and cytoplasm. (C) SIRT3, (D) SIRT4 and (E) SIRT5 were primarily mitochondrial proteins. (F) SIRT6 and (G) SIRT7 were mainly nuclear Sirtuins. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: IF in TNF-α-stimulated HaCaT cells was negative except for SIRT6 and 7. (A) SIRT1 and (B) SIRT2 were located predominantly in the nucleus and cytoplasm. (C) SIRT3, (D) SIRT4 and (E) SIRT5 were primarily mitochondrial proteins. (F) SIRT6 and (G) SIRT7 were mainly nuclear Sirtuins. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Article Snippet: Membranes were subsequently blocked with 5% non-fat milk for 1 h at room temperature and probed with specific antibodies overnight at 4°C against SIRT1 (1:600; cat. no. 13161-1-AP; ProteinTech Group, Inc.), SIRT2 (1:600; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd.), SIRT3 (1:600; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:200, cat. no. ab10140; Abcam), SIRT5 (1:600; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:600; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.), SIRT7 (1:600; cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.), GAPDH (1:2,000; cat. no. GB11002; Wuhan Servicebio Technology Co., Ltd.) and β-actin (1:1,000; cat. no. ab8226; Abcam).

Techniques: Immunofluorescence